Determination of 1,1,1,2,3,3,3-heptafluoropropane (HFP) in blood by headspace gas chromatography-mass spectrometry.

نویسندگان

  • Volker Auwärter
  • Hans Proquitté
  • Gerd Schmalisch
  • Roland Wauer
  • Fritz Pragst
چکیده

The inert and certified medical gas 1,1,1,2,3,3,3-heptafluoropropane (HFP) is commonly used as a propellant in medical sprays. HFP is also of interest as tracer gas for lung function measurements. In this work a sensitive method for quantitative determination of HFP in blood was developed using headspace gas chromatography-mass spectrometry with N2 as internal standard. The limits of detection and quantification were 0.005 and 0.016 mg/L, respectively. The method was applied to blood samples from newborn piglets that were ventilated with air containing 0.8% HFP (v/v) for 30 min. This was done to assess accumulation of HFP during measurement of functional residual lung capacity by wash-in and wash-out techniques. A saturation concentration in blood between 2.0 and 2.5 mg/L was attained after 3 min ventilation and decreased with a half-life below 1 min after termination of the HFP supply. No relevant accumulation of HFP was observed.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Simultaneous Determination of Disinfection By-products in Water Samples from Advanced Membrane Treatments by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry

A headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) method has been developed for the simultaneously determination of 20 disinfection by-products (DBPs) in water samples from reverse osmosis (RO) membranes. Selected compounds belong to different families including: trihalomethanes (THMs), halogenated acetonitriles (HANs), halogenated nitromethanes ...

متن کامل

Identification of volatile organic compounds of some Trichoderma species using static headspace gas chromatography-mass spectrometry

Fungi release wide spectrum of volatile organic compounds (VOCs) that belong to several chemical groups with different biochemical origins such as monoterpenes, sesquiterpenes, alcohols, aldehydes, aromatic compounds, esters, furans, ketones, sulfur and nitrogen compounds. Trichoderma species are the most studied fungal biocontrol agents and are successfully used as biofungicides and biofertili...

متن کامل

Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry.

A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-(13)C(3) as an internal standard. The developed me...

متن کامل

Microanalysis of carbon monoxide in decomposed blood and hepatic tissues by headspace gas chromatography and mass spectrometry

Head-Space Gas Chromatography-Mass Spectrometry (HS-GC/MS) was used to determine blood carboxyhemoglobin (COHb%), especially in cases involving blood decomposition or death, when blood extraction is impossible. Until now, the problem eluded complete solution using forensic toxicology. Therefore a method was developed using Headspace Gas Chromatography-Mass Spectrometry (HSGC/MS), which should e...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of analytical toxicology

دوره 29 6  شماره 

صفحات  -

تاریخ انتشار 2005